rpb1 ntd Search Results


rnapolii ser5p cst  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rnapolii ser5p cst
A: IGV snapshot depicting ChIP profiles of Mll-N, H3K4me3, RNAPolII (total), <t>RNAPolII-Ser5P,</t> and RNAPolII-Ser2P at the promoter of the Set target Med23 . B: Metagene plots across all Set activated genes reveal a specific loss of Mll and elongating RNA polymerase from chromatin after Set depletion. C: Only promoter recruited PP2A can repress the MLL responsive human Meis1 promoter. The reporter construct was co-transfected with expression constructs as indicated and luciferase results were determined in triplicate experiments. D: Recruitment of Set and PP2A to the endogenous Meis1 promoter in myeloid precursor cells. Effectors were targeted to the murine Meis1 promoter in MLL-ENL transformed cells by a fusion with catalytically inactive Cas9 (dCas9) in combination either with a non-targeting (vec) or two Meis1 promoter specific sgRNAs. Meis1 output was measured by RT-qPCR in triplicates.
Rnapolii Ser5p Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rpb1+ntd/Rpb1+NTD+Rabbit+mAb/bio_rxiv__64898__2026__02__26__708410-157-27-28
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rnapolii ser5p cst - by Bioz Stars, 2026-10
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A: IGV snapshot depicting ChIP profiles of Mll-N, H3K4me3, RNAPolII (total), RNAPolII-Ser5P, and RNAPolII-Ser2P at the promoter of the Set target Med23 . B: Metagene plots across all Set activated genes reveal a specific loss of Mll and elongating RNA polymerase from chromatin after Set depletion. C: Only promoter recruited PP2A can repress the MLL responsive human Meis1 promoter. The reporter construct was co-transfected with expression constructs as indicated and luciferase results were determined in triplicate experiments. D: Recruitment of Set and PP2A to the endogenous Meis1 promoter in myeloid precursor cells. Effectors were targeted to the murine Meis1 promoter in MLL-ENL transformed cells by a fusion with catalytically inactive Cas9 (dCas9) in combination either with a non-targeting (vec) or two Meis1 promoter specific sgRNAs. Meis1 output was measured by RT-qPCR in triplicates.

Journal: bioRxiv

Article Title: The nuclear oncoprotein SET is necessary for MLL/KMT2A binding and transcriptional elongation

doi: 10.64898/2026.02.26.708410

Figure Lengend Snippet: A: IGV snapshot depicting ChIP profiles of Mll-N, H3K4me3, RNAPolII (total), RNAPolII-Ser5P, and RNAPolII-Ser2P at the promoter of the Set target Med23 . B: Metagene plots across all Set activated genes reveal a specific loss of Mll and elongating RNA polymerase from chromatin after Set depletion. C: Only promoter recruited PP2A can repress the MLL responsive human Meis1 promoter. The reporter construct was co-transfected with expression constructs as indicated and luciferase results were determined in triplicate experiments. D: Recruitment of Set and PP2A to the endogenous Meis1 promoter in myeloid precursor cells. Effectors were targeted to the murine Meis1 promoter in MLL-ENL transformed cells by a fusion with catalytically inactive Cas9 (dCas9) in combination either with a non-targeting (vec) or two Meis1 promoter specific sgRNAs. Meis1 output was measured by RT-qPCR in triplicates.

Article Snippet: Set : Invitrogen (#MA5-35772); MLL: CST (#8178) mixed 1:1 with anti-MLLN, Upstate (Temecula, CA, #05-764) 10 μl AB-mix per 5×10e6 cells, H3K4me3: CST (#9751), RNAPolymeraseII: CST (#14958), RNAPolII-Ser5P: CST (#13499), RNAPolII-Ser2P: CST (#13523), Msk1-P (Ser376P): CST (#9591), H3S10P: CST (#53348), H3K9ac: CST (#9649), H3K14ac: CST (#7627), H3K27ac: CST (#8173), Cbp: CST (#7389), Creb-P (Ser133P): CST (#9198), MOZ: Invitrogen (PA5-68046) mixed 1:1 with Invitrogen (PA5-103467), HA: CST (#3724), flag: Merck (F1804).

Techniques: Construct, Transfection, Expressing, Luciferase, Transformation Assay, Quantitative RT-PCR